Feel free to contact us
for any questions or custom requests!
Key Benefits
– Filter all 96 wells at once by centrifugation
– Minimize inter-well cross-contamination with a contamination-preventive silicone sheet that securely fixes plates.
– Ideal for removing cell clumps and debris, and preparing single-cell before flow cytometry.
– Available pore sizes: 30 μm / 70 μm / 100 μm.
Payment by credit card is available. Get a quote today.
In field where flow cytometers are used, the following problems occur with conventional method using cell strainers.
– Filtration is a pain
– Need much time and efforts
– Total cost is high
To solve such problems, we developed and sell this “Centrifugal filtration plate” and “96-well compatible microplate mesh seal“!
The CSCRIE 96-well sample filtration plate is a high-throughput mesh filter plate, designed for efficient filtration of various samples in standard 96-well microplates.
– Can process multiple samples efficiently
– Prevent contamination per well
– Unit cost of experiment (filtration cost) can be reduced
Highly homogeneous mesh is used, with pore size 30, 70 and 100 μm.
(1) Stack our centrifugal filtration plate onto reservoir microplate (compatible with standard 96-well plates)
(2) Apply samples to all wells by using pipettes
(3) Filtration with centrifuge *Recommended: 300g x 30 seconds
This video demonstrates how to use the CSCRIE 96-well filtration plate for centrifugation-based sample filtration.
Before cell filtration
After cell filtration
Set a centrifugal filtration plate on a conventional 96-well round bottom plate and pass cells through filter → centrifugation
Evaluation point: Presence or absence of contamination after plate centrifugation
Presence or absence of contamination
HEK293T not expressing GFP and HEK293T expressing GFP (human fetal kidney epithelial cell) are filtered in adjacent wells and judged by flow cytometry
① Cells were stripped with trypsin/EDTA and centrifuged.
② Cells were washed with PBS.
③ Cells were diluted with PBS to make about 2 x 104 cells/200 μL.
④ PI was added to make a final concentration of 0.25 μg/mL.
⑤ A centrifugal filtration plate was set on a 96-well plate.
⑥ Cells were added to wells in 200 μL.
⑦ The plate was centrifuged at 300 g x 30 s.
⑧ It was measured with flow cytometer (Guava easyCyte 8HT) .
*Measurement was made only within the red frame.
It is being put in a centrifugal filtration plate
There was no suspension left in the filter.
After centrifugation
・After centrifugation, there was no contamination in another well.
・This time, it was centrifuged at 300 g x 30 seconds, and all the suspension had fallen to the bottom.
| Product name | CSCRIE Centrifugal Filtration Plate |
|---|---|
| Standard | Compatible with 96-well plate |
| Materials | Plate : Polystyrene Mesh : Polyester fiber Adhesive : Silicone |
96 well type
| Product Code | Description | Q’ty | Price |
| R-CSC078-02 | CSCRIE Centrifugal Filtration Plate, 96 well, 30 µm | 10 plates | Contact us |
| R-CSC079-02 | CSCRIE Centrifugal Filtration Plate, 96 well, 70 µm | 10 plates | |
| R-CSC080-02 | CSCRIE Centrifugal Filtration Plate, 96 well, 100 µm | 10 plates |
Payment by credit card is available. Get a quote today.
A. It is compatible with a standard 96-well plate.
A. 30 μm, 70 μm, and 100 μm are available as off-the-shelf products. Custom pore size is also available, feel free to contact us.
A. Try 300 g x 30 seconds first, but please optimize the condition according to your experiments.
A. A contamination-preventive silicone sheet (patented technology) securely fixes both plates.
A. Yes. Customization is available. Contact us.
A. Please contact us for a quote. Credit card payment is available.
Our extensive knowledge and expertise enable us to make customized filtration microplate with the pore size range from 0.01 to 300 μm.
Please feel free to contact us!
Designed for high througput sample filtration on 96 well plate.
Designed for high througput sample filtration from 384-well plate by centrifuge.
